Figure 3C AJ 161125

This dataset comprises TIF files representing acquisitions in one single molecule experiment. There are two types of file. Each TIF file contains a stack of images representing a time series at a single field of view. An accompanying text file indicates the laser in use at each frame. For example, in the first file, the exposure time for each frame was 100 msec. Illumination was by laser 2 (640 nm, Cy5) from time frames 1 to 48, laser 3 (561 nm, mCherry) from frames 50-248 and laser 1 (488 nm, mEGFP) from frames 103 to 242. Deviation (Dev) represents the deviation of the exiting laser as a result of autofocussing from the point at which all four quarters of a quartered photodiode were irradiated with equal intensity. The experiment was done by Andrew Jobbins.

本数据集包含代表单一分子实验获取的 TIF 文件。数据集包含两种类型的文件。每个 TIF 文件包含一系列图像，这些图像表示单一视场中的时间序列。随附的文本文件指示每个帧所使用的激光类型。例如，在第一个文件中，每帧的曝光时间为 100 毫秒。照明由激光 2（640 纳米，Cy5）在时间帧 1 至 48 之间提供，激光 3（561 纳米，mCherry）在帧 50 至 248 之间提供，以及激光 1（488 纳米，mEGFP）在帧 103 至 242 之间提供。偏差（Dev）表示由于自聚焦导致的激光输出从四个象限的 photodiode 均匀照射点偏移的程度。该实验由 Andrew Jobbins 完成。