RT quantitative PCR analysis of p53 Signaling Pathway related genes in mice choclea after noise exposure.. RT quantitative PCR analysis of p53 Signaling Pathway related genes in mice choclea after noise exposure.

Six month-old wild type and Igf1 heterozygous mice were exposed to a violet swept sine noise (frequency range 2–20 kHz) at 110 dB SPL for 30 minutes. Cochlear samples were taken at 2 and 24 hours post-noise exposure to study the evolution of the expression of inflammation genes by using PCR arrays and RT-qPCR. Six month-old wild type and Igf1 heterozygous mice not exposed to noise were used as control. Overall design: qPCR gene expression profiling. Cochlea samples from 3 animals per group were analised. The expression of genes related to inflammation was studied at 2 and 24 hours post-noise exposure.