Transcriptome analysis of wild-type and Yap-deficient embryonic lungs by RNA-Seq

We extracted total RNA from wild-type and Yap-f/f;Shh-Cre/+ embryonic lung 12.5 dpc, and perform RNA-Seq. mRNA profiles of embryonic lungs from wild type and Yap mutant littermates at 12.5 dpc were generated by deep sequencing in triplicate.我们从野生型（wild-type）及Yap-f/f;Shh-Cre/+基因型的妊娠后12.5天（days post coitum, dpc）胚胎肺中提取总RNA，并开展RNA测序（RNA-Sequencing, RNA-Seq）实验。针对该发育阶段的野生型与Yap突变型同窝仔鼠胚胎肺，本研究通过深度测序（deep sequencing）设置三次生物学重复，以获取其mRNA表达谱。