Regulation of Tfh differentiation by Mettl3 [RNA-seq]

T follicular helper (Tfh) cells are a specialized subset of CD4 T cells that are essential for most antibody productions and humoral responses. It is well characterized that transcriptional network exerts indispensable roles in Tfh cell differentiation. However, how post-transcriptional regulation controls Tfh biology has largely remained unclear. Multiple mice models were used here by a series of experimental approaches to illustrate m6A methyltransferase Mettl3 programs Tfh cell differentiation at post-transcriptional layer. Overall design: Ctrl and Mettl3-null Tfh cells (3 samples per group) or Th1 cells (2 samples per group) were sorted at day 8 after LCMV Armstrong infection. Then the total RNAs were extracted and subjected to RNA sequencing.