Escherichia coli from dairy cattle farms

The aim of this research was to detect the frequency of shiga toxigenic Escherichia coli isolates among various samples from three dairy farms: diarrheic and non-diarrheic fecal samples, cattle urine samples, swabs from milkers' hands, and water samples. And to characterize Shiga toxins and their subtypes. Added to that to make sequence analysis among one of the shiga toxin genes from cattle and milker hands isolates. Moreover, to detect phenotypic with genotypic antimicrobial resistance and discrepancies between genotype-phenotype of extended-spectrum beta lactamases and tetracycline resistance. To detect the profiles of virulence and antimicrobial resistance genes. To elucidate the genetic relationship and diversity of Escherichia coli isolates from various samples.