Screening of circular-permutated mCherry variants in E.coli.

*Circular-permutated mCherry variants were numbered by the primary amino acid sequence of mCherry as shown in Fig. 1C. Variants are labeled with the new amino and carboxy termini (e.g. cp159-158 has the new N-terminus from the native carboxy amino acids 159 to 236 and the new C-terminus from the amino residues 1 to158).aFluorescent variants from the initial screening in each region.bAligns to cp-EGFP in GCaMP2.cNumbered by the amino acid sequence of DsRed as shown in Fig. 1C[18], [19]. cp184-mCherry corresponds to cp191-190, with three duplicated residues at N-terminus (188-190). The cp193-mCherry and cp193g7 (with 6 mutated amino acids) correspond to cp197-196, with three duplicated residues (197-199) at the C-terminus.