Supplement 2. Next generation sequencing results for first round screen from CRISPR Genome-Wide Screening Identifies Dependence on the Proteasome Subunit PSMC6 for Bortezomib Sensitivity in Multiple Myeloma

RPMI8226Cas9 cells were infected with the CRISPR library. The infected cells were treated with BTZ at 7nM for seven days (changed medium with 7 nM BTZ at day 2 and day 4). Residual cells surviving BTZ treatment were expanded over 15 days without BTZ treatment and then subjected to DNA sequencing. Experimental screening procedures are illustrated in figure 2A. The top 100 sgRNAs that featured in next generation sequencing of surviving cells following BTZ treatment are listed.