miR-7977 transfer alter the transcriptome in bone marrow mesenchymal stem cells.

Acute myeloid leukemia (AML) cells release abundant exosomal miR-7977 that transfer into bone marrow (BM) mesenchymal stromal cells (MSCs). We have shown that exosomal miR-7977 was highly released from AML cells and was transferred into BM MSCs. It has been well known that a microRNA has multiple targets. In fact, miRDB predicted 633 targets. Based on these findings, control and miR-7977mimic were transferred into BM MSCs. Subsequently, alteration of transcriptome was analyzed to gain insight into the role of miR-7977 in bone marrow micro environment. We performed transduction of control or miR-7977 mimic into MSCs, compared transcriptome in between control transduced (n=3) and miR-7977 transduced MSCs (n=3) using the Affymetrix Human Gene 2.0 ST platform.