ZEB1 is regulated by K811 acetylation to promote stability, NuRD complex interactions, EMT, and NSCLC metastasis. ZEB1 is regulated by K811 acetylation to promote stability, NuRD complex interactions, EMT, and NSCLC metastasis

To understand the role of Zeb1 acetylation in regulating its function a Zeb1 acetyl-mimetic and acetyl-deficient mutant expressing NSCLC cell line was generated. We then performed gene expression profiling analysis using data obtained from RNA-seq of GFP-vector, Zeb1 wild-type, Zeb1 deficient, and Zeb1 mimetic. Overall design: Comparative gene expression profiling analysis of RNA-seq data for 393P cells with GFP-vector (negative control), Zeb1-WT (wild-type), Zeb1_deficent, Zeb1_mimetic, three different samples submitted, 2 replicates per sample per cell line.