LC-MALDI MS-based identification of proteins from membrane cavitates from capacitated porcine sperm.

Presented are subsets of those proteins identified that correspond to previously identified sperm proteins relevant to sperm-oocyte interactions [29]. SNARE-related protein identifications are highlighted in bold. In addition one proteins with a putative function in the recruitment of SNARE proteins into membrane rafts [26] is shown in red. Number signs indicate proteins that have been previously found in membrane cavitates of porcine sperm [29]. Asterisks indicate proteins that were identified in the MV preparations (Tables 1 and 2). Protein ID was considered to be conclusive when two or more peptides were identified (eProtein ID was considered conclusive when two or more peptides were identified (eHighlighted in bold: SNARE proteins or SNARE interacting proteins.Highlighted in red: Protein with putative function in the recruitment of SNARE proteins into the membrane raft area.#: indicate proteins which are routinely found in the apical plasma membrane cavitates of porcine sperm.*: indicate proteins that are recovered as plasma membrane proteins in the mixed vesicles.