High occurrence of jellyfish predation by black-browed and Campbell albatross identified by DNA metabarcoding

This spreadsheet provides the sequences counts for the DNA groups found in the scats of black-browed albatross at New Island and Steeple Jason Island, Falkland Islands; Diego Ramírez and Albatross Islet, Chile; Bird Island, South Georgia; Canyon des Sourcils Noirs, Kerguelen Archipelago, France; Macquarie Island, Australia; and Campbell albatross at Campbell Island, NZ.Scat samples were collected in 2013/14 and 2014/15 at New Island, Steeple Jason Island, Macquarie Island, Campbell Island and Bird Island; in 2013/14 and 2015/16 at Kerguelen; in 2014/15 and 2015/16 at Albatross Islet and in 2013/14 at Diego Ramírez. Samples were collected during Incubation (Oct-Nov), early chick-rearing (Dec-Jan) or late-chick rearing (Feb-Mar). Due to the availability of birds at the colony, samples were predominantly collected from adults during incubation and early chick-rearing and chicks during late chick rearing. Samples sizes were too low during this study to directly compare dietary differences between chicks and adults; however, dietary comparisons between breeding stages were examined for sites where samples were collected during multiple breeding stages.Samples were PCR amplified with a universal metazoan primer set that is highly conserved and amplifies a region of the nuclear small subunit ribosomal DNA gene (18S rDNA). Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Raymond, B., Lea, M-A., Deagle, B., Catry, P., Gras, M., Phillip, R.A., Stanworth, A., Suazo, C., Thompson, D., Weimerskirch, H., Gras. M., and Jarman, S.N. High occurrence of jellyfish predation by black-browed and Campbell albatross identified by DNA metabarcoding. Molecular Ecology.

本数据集表格提供了福克兰群岛新岛（New Island）与陡杰森岛（Steeple Jason Island）、智利迭戈拉米雷斯岛（Diego Ramírez）与信天翁岛（Albatross Islet）、南乔治亚州鸟岛（Bird Island）、法国凯尔盖朗群岛黑源峡谷（Canyon des Sourcils Noirs）、澳大利亚麦夸里岛（Macquarie Island）的黑眉信天翁粪便，以及新西兰坎贝尔岛的坎贝尔信天翁粪便中检出的DNA类群序列计数数据。
粪便样本采集时间如下：新岛、陡杰森岛、麦夸里岛、坎贝尔岛与鸟岛的样本采集于2013/14及2014/15年度；凯尔盖朗群岛的样本采集于2013/14及2015/16年度；信天翁岛的样本采集于2014/15及2015/16年度；迭戈拉米雷斯岛的样本仅采集于2013/14年度。
样本采集时段涵盖繁殖期（10-11月）、雏鸟早期抚育期（12-1月）与雏鸟晚期抚育期（2-3月）。
由于繁殖种群中鸟类的可及性差异，孵化期与雏鸟早期抚育期的样本主要取自成鸟，而雏鸟晚期抚育期的样本则主要取自雏鸟。
本研究的样本量较小，无法直接对比雏鸟与成鸟的饮食差异；但针对多个繁殖阶段均有样本采集的研究站点，本数据集对不同繁殖阶段的饮食差异进行了分析。
本研究采用高度保守的通用后生动物引物组进行聚合酶链式反应（PCR）扩增，目标片段为核小亚基核糖体DNA基因（18S rDNA）的特定区域。
本分子实验方法与数据集合成的详细信息可参见以下文献：McInnes, J.C.、Alderman, R.、Raymond, B.、Lea, M-A.、Deagle, B.、Catry, P.、Gras, M.、Phillip, R.A.、Stanworth, A.、Suazo, C.、Thompson, D.、Weimerskirch, H.、Gras, M. 与 Jarman, S.N. 发表于《分子生态学》的论文《通过DNA元条形码（DNA metabarcoding）技术鉴定黑眉信天翁与坎贝尔信天翁的高水母捕食率》。