Descriptor Somalogic proteome expression

EDTA blood samples were collected from participants, cells were centrifuged, and supernatants and pellets were stored at -80 o C until analysis. Plasma was centrifuged for 15 min at 2200 x g, and 60 ul of supernatant was used for used for the SOMAscan assay performed by SomaLogic, Boulder, CO as described previously (Gold et al., 2010;Han et al., 2018;Tin et al., 2019;Yang et al., 2020). Raw signals were then normalized as described (Gold et al., 2010;Han et al., 2018). These steps include hybridization normalization, plate scaling and calibration, and the adaptive normalization by maximum likelihood (ANML), which normalizes SomaScan EDTA plasma measurements to a healthy U.S. population reference, and then log2 transformed, (resulting in data file: sbst3_norm_SIG_Somalogic_UTHSC_2021_291122.txt.The descriptor of the participants is provided here as table sbst3_target_SIG_proteome_Soma_251122a.xlsx