A pair of microRNA sequencing datasets for the same set of sarcoma samples. Homo sapiens

We sequenced tumor samples for the two most common and aggressive subtypes of genetically complex soft tissue sarcoma: (1) myxofibrosarcoma (MXF); (2) pleomorphic malignant fibrous histiocytoma (PMFH) twice.o In the first study, library preparation and read capture were each handled by a single experienced technician in one run. In addition, sample-to-library allocation used blocking and randomization, so that, if there are still any residual handling effects (despite the use of uniform handling) they are balanced between the two sample-groups and hence can cancel out in the differential expression analysis. That is, each library used 20 unique barcodes that were previously validated, allowing 20 samples to be included in a library (38); three libraries were used to sequence the 54 individual tumor samples and two pooled samples (one from pooling the 27 MXF samples and the other from pooling the 27 PMFHs); each library included 9 MXFs, 9 PMFHs, the pooled MXF, and the pooled PMFH. To add extra rigor for ensuring data quality, we also included ten calibrators with fixed input concentrations for each sample and duplicated the entire experiment.o In the second study, the same 54 tumors were sequenced over three years by multiple technicians in the order of sample collection. RNAs used for sequencing runs of the same sample were derived from the same cryomold; care was taken to ensure consistent sample handling for the two studies. For quality-control, the same ten calibrators were used as spiked-ins.