Figure 2

Figure 2. Viral shedding and lung lobe viral load of WT and NA-H275Y+PA-I38T virus isolate infected ferrets. Ferrets (n=8) infected by the intranasal route with 4 log10TCID50/mL (in 500 µL of PBS) with WT (d1 isolate, black) or NA-H275Y+PA-I38T (d10 isolate, red) purified virus isolates were nasally washed daily for 9 days. Half of the ferrets were culled at day five post-inoculation to harvest the five major lung lobes. (a) The viral shedding from the ferret nasal washes was measured by TCID50 assay. (b) Viral titre of each homogenised lung lobe aliquot wase enumerated using a TCID50 assay, the fraction representing the number of lobes with detectable viral titres (above LOD) per ferret.