Vibrio fischeri D-alanine suppressor generation and characterization

This study used D-alanine auxotrophic mutants of Vibrio fischeri to study the evolution of peptidoglycan biosynthesis and structure. Suppressor mutants were generated by growing an auxotrophic strain without necessary exogenous D-alanine. Mutants were then characterized and genome sequenced, in order to determine the mechanism of suppression.