Additional file 10: of Expanding the biodiversity of Oenococcus oeni through comparative genomics of apple cider and kombucha strains

Table S3. Primer list. The sequence surrounding NNN-islands in the CRBO_1381 assembly scaffold was entered into Primer3 with default settings (GC clamp = 1) to find suitable primersets for PCR product sequencing. The target product size, discounting Ns, was 1 kb. Primersets were tested with Primer-BLAST on PSU-1. PCR product size was tested by agarose gel and multiNA and sequenced by Eurofins Genomics (XLSX 5 kb)附表S3 引物列表。将CRBO_1381组装支架中NNN岛（NNN-islands）的侧翼序列输入至Primer3软件，采用默认参数（GC钳位（GC clamp）=1）筛选适用于PCR产物测序的引物对。去除N碱基后的目标产物长度为1 kb。引物对通过Primer-BLAST在PSU-1上进行特异性验证。PCR产物长度通过琼脂糖凝胶电泳与multiNA进行检测，测序工作由Eurofins Genomics完成（附件为大小5 KB的XLSX文件）