Bioenergetic control of synaptic plasticity by astrocytes during acute stress
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https://www.ncbi.nlm.nih.gov/sra/SRP184236
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Aldh1l1CreERT2-RiboTag mice were used to purify and sequence astrocyte actively translated mRNAs from acute stress mouse models (20 minute forced swim) and non stressed controls. Overall design: Four weeks after tamoxifen injection, somatosensory cortex was homogenized and RNA was purified from (i) cleared lysate as the input and control, and (ii) astrocyte-specific ribosome-associated RNA precipitated via a hemagglutinin (HA) tag. Stress mouse astrocyte transcriptomes were compared to non stressed.
创建时间:
2020-05-08



