Influence of cultivar, fungal exudate fractions and zinniol on cell suspension integrity and somatic embryogenesis.

Carrot cell suspensions with six different genotypes were tested for embryogenesis in the presence of fungal extracts and toxins. Embryogenesis was assessed 3 weeks after treatment.1Treatments were as follows: rA: Alternaria dauci (strain FRA017) fungal culture raw extract; rM: uninoculated medium raw extract; aA: A. dauci fungal culture aqueous extract; aM: uninoculated medium aqueous extract; oA: A. dauci fungal culture organic extract; oM: uninoculated medium organic extract; DMSO: DMSO solution, at a concentration corresponding to oM, z1, z2 and z3 treatments; z1: 0.025 µM zinniol; z2: 10 µM zinniol; z3: 500 µM zinniol. C: no treatment.2The signs are as follows: (−) no embryogenesis was visible and cells were damaged, (+) early-stage embryogenic masses were visible, (++) embryos were present, (+++) embryogenesis was profuse. +/− early-stage embryogenic masses were visible, or no embryogenesis was visible depending on the repetition.