Additional file 10 of Detection and discovery of plant viruses in soybean by metagenomic sequencing
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Additional file 10 Figure S10. Mixed infections of viruses confirmed by Sanger sequencing in field-grown soybean plants. Reverse transcription-polymerase chain reaction (RT-PCR) was performed in all samples identified with mixed infections. The PCR products were visualized in a 1% agarose gel electrophoresis containing SYBR Safe DNA gel stain. The PCR products were cleaned using ExoSAP-IT PCR product cleanup reagent and sequenced on Sanger sequencing platform. Primer sequences were designed using Primer3 plus from assembled contigs and subsequently used in this study
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Kleczewski, Nathan M.; Sikora, Edward; Whitham, Steven A.; Hajimorad, M. R.; Stewart, Tracey P.; Smith, Damon L.; Mueller, Daren S.; Gaskill, Mikaela A.; Elmore, Manjula G.; Wise, Kiersten A.; Groves, Carol L.
创建时间：
2022-09-14
