Additional file 10 of Detection and discovery of plant viruses in soybean by metagenomic sequencing

Additional file 10 Figure S10. Mixed infections of viruses confirmed by Sanger sequencing in field-grown soybean plants. Reverse transcription-polymerase chain reaction (RT-PCR) was performed in all samples identified with mixed infections. The PCR products were visualized in a 1% agarose gel electrophoresis containing SYBR Safe DNA gel stain. The PCR products were cleaned using ExoSAP-IT PCR product cleanup reagent and sequenced on Sanger sequencing platform. Primer sequences were designed using Primer3 plus from assembled contigs and subsequently used in this study