Verification of CRISPR editing and finding transgenic inserts by Xdrop™ Indirect sequence capture followed by short- and long- read sequencing

Two reconstructed APOE reference loci on GRCh38.p13 Chr19:44,900,000-44,920,000 including corresponding pEasy Flox fragments of 3.4 kb and 4.4 kb. A reconstructed Pax8-CreERT2 reference loci on GRCm38.p6 chr1: 93570239-93676772.两个经重构的载脂蛋白E（APOE）参考基因座，定位于人类参考基因组GRCh38.p13的19号染色体44,900,000-44,920,000区间，附带对应的3.4 kb与4.4 kb的pEasy Flox片段。一个经重构的Pax8-CreERT2参考基因座，定位于小鼠参考基因组GRCm38.p6的1号染色体93570239-93676772区间。