Genome-wide Transcriptome Analysis of CD36 Overexpression in HepG2.2.15 Cells to Explore Its Regulation Role of Metabolism and HBV Life Cycle

To understand the molecular basis underlying the response to increasing CD36 overexpression in HepG2.2.15 cell, we performed genome-wide sequencing of the mRNAs from HepG2.2.15-CD36OE cell and HepG2.2.15-vector cell using the RNA-seq technology to detect its differential transcriptomic profile. Overall design: To better understand the molecular changes in the response of the CD36 overexpression in HepG2.2.15 cell, three cDNA libraries from HepG2.2.15-vector cell (Vector-1, Vector-2, Vector-3) and HepG2.2.15-CD36 overexpression cell (CD36OE-1, CD36OE-2, CD36OE-3) were constructed, respectively. After ensuring quality control, Ion ProtonTM Sequencer was used for sequencing the cDNA libraries.