Priming-induced changes in germination, morpho-physiological and leaf biochemical responses of fenugreek (<i>Trigonella foenum-graecum</i>) under salt stress

Seed priming is a simple biotechnological tool which is potentially able to promote seed germination and invigoration as well as seedlings establishment and stress tolerance. In this study, the effects of seed pre-treatment with water (hydro-priming), 100 (HP-NaCl₁₀₀) and 200 mM (HP-NaCl₂₀₀) NaCl (halo-priming) for 6 h on some physiological and biochemical parameters of fenugreek was investigated under saline conditions (100 mM NaCl). For the three priming treatments, no significant changes in the final germination percentage were observed. However, a decrease in seed germination time was observed in hydro- and halo-primed (HP-NaCl₂₀₀) seeds. Salt stress (100 mM NaCl) reduced growth (shoot and root dry weight), pigment content, disturbed the ionic balance and enhanced malondialdehyde content. Salinity-induced changes in lipid metabolism towards synthesis/accumulation of saturated and monounsaturated fatty acids were observed in stressed plants. Seed priming ameliorated the negative effects of NaCl, ensuring significant amelioration on growth, pigment content, increased the activity of catalase and glutathione peroxidase and enhanced the synthesis of very long chain <i>n</i>-alkanes. Taken together, these data provide compelling evidence that priming is an effective alternative that can be used to promote germination and improves establishment and acclimation of fenugreek seedlings under saline conditions.

种子引发（seed priming）是一种简易的生物技术手段，可有效促进种子萌发与活力提升，同时助力幼苗建成并增强其抗逆性。本研究以胡芦巴为材料，设置清水引发（hydro-priming）、100 mM（HP-NaCl₁₀₀）与200 mM（HP-NaCl₂₀₀）氯化钠盐引发（halo-priming）三种预处理方式，处理时长为6小时，在100 mM NaCl的盐胁迫条件下，探究其对胡芦巴部分生理生化指标的影响。三种引发处理均未使最终发芽率产生显著变化。不过，清水引发与200 mM氯化钠盐引发（HP-NaCl₂₀₀）的种子，其萌发时间有所缩短。盐胁迫（100 mM NaCl）会抑制植株生长（地上部与根部干重）、降低色素含量，扰乱离子平衡并提升丙二醛含量。盐胁迫还会诱导植株脂质代谢向饱和与单不饱和脂肪酸的合成/积累方向转变。种子引发可缓解NaCl胁迫带来的负面影响，显著改善植株生长与色素含量，提升过氧化氢酶与谷胱甘肽过氧化物酶的活性，并促进超长链<i>n</i>-alkanes的合成。综上，本研究数据充分证明，种子引发是一种高效可行的替代手段，可用于促进盐胁迫环境下胡芦巴的种子萌发，提升幼苗建成与驯化适应能力。

搜集汇总

背景与挑战

背景概述

该数据集研究了预处理（水合及不同浓度NaCl处理）对葫芦巴种子在盐胁迫下萌发、生长和生理生化响应的影响。实验发现预处理虽未显著改变最终萌发率，但缩短了萌发时间，并缓解了盐胁迫导致的生长抑制、色素含量下降及氧化损伤，同时增强了抗氧化酶活性和脂质代谢适应性。结果表明预处理是提高葫芦巴幼苗在盐渍条件下适应性的有效方法。