ChIP-Seq of mouse mammary gland tissues

To sort out the factors that might be negatively regulated by Brca1 but potentiated by ERα in the proliferating mammary epithelial cells, we investigate transcriptional activities of promoters genome-wide using antibodies against H3K4me3, H3K27Ac, Brca1, and estrogen alpha (ERα) by performing chromatin immunoprecipitation (ChIP) sequencing in primary mammary epithelial cells isolated from MTP12 and WTP12 mice ChIP sequence for mammary gland tissues isolated from both Brca1 wild type (3XFlag-Brca1+/+) and mutant mice (Brca1co/co Trp53+/- MMTV-Cre mice) at 12.5 (P12) days of pregnancy by using Flag-Brca1, ERα, H3K4me3, and H3K27Ac.