Administration of soluble Robo4 (sR4) shifts the Robo4 knockout to the WT phenotype.

WT and Robo4 knockout mice (R4 KO) were infected with HSV-1 RE. (A) R4 KO mice received either sR4 (R4 KO treated w/ sR4) or vehicle (R4 KO Control) from 2 to 14 days pi. WT mice were included and received vehicle (WT Control) under the same regimen previously stated. (B) SK lesions and angiogenesis severity was decreased in R4 KO mice treated with sR4. (C) Representative eye photos show that R4 KO mice treated with sR4 do not develop the severe phenotype that R4 KO control animals do (D) Hematoxylin and eosin staining was carried out on 6-μm sections, and pictures were taken 40 x magnification. Representative eye sections show decreased cellular infiltration in R4 KO treated w/ sR4 and WT control compared to R4 KO control mice. Data are representative of two independent experiments and show mean values ± SEM (n = 12 mice/group). ***p ≤ 0.001,**p ≤ 0.01,*p ≤ 0.05. Statistical levels of significance were analyzed by one-way ANOVA test with Tuckey’s post hoc test settings.