Efficient gene knockout and genetic interaction screening using the in4mer CRISPR/Cas12a multiplex knockout platform

Figure 1: Meta-analysis of synthetic lethality screens. Figure 2: Multiplexing beyond 2 guides with enAsCas12a. Figure 3: In4mer platform for whole-genome screening Figure 4: Prototype and Inzolia screens Figure 5: Synthetic chemogenetic interactions Figure 6: Library size comparison Supplementary Table 1: dLFC and Cohen's D for five published paralog studies Supplementary Table 2: 388 Identified synthetic lethals, 26 synthetic lethals and gold standard set Supplementary Table 3: Raw read counts for 7mer screens Supplementary Table 4: Prototype library sequence Supplementary Table 5: Inzolia library sequence Supplementary Table 6: Raw read counts for both Prototype and Inzolia library screens Supplementary Table 7: Log fold change by gene for both Prototype and Inzolia library screens Supplementary Table 8: DrugZ score for MELJUSO screen with MEK inhibitor selumetinib Supplementary Table 9: GSEA result for MELJUSO screen with MEK inhibitor selumetinib