Table S1 - Pix Proteins and the Evolution of Centrioles

Survey across eukaryotes of centrioles, cilia, flagellum-specific β-tubulin and Pix homologues. Deuterostomia (white), Ecdysozoa (blue), Lophotrochozoa (grey), Cnidaria (yellow), Fungi (pink), plants and protistan sister groups (green), other protists (purple). Column 3, taxonomic groups are from the NCBI taxonomic database. The β-tubulin cilia/flagellum C-terminal domain was sought in genomes using BLAST with the Drosophila sequence (EGEFDED; the human sequence is EGEFDEE and consensus is EGEF+3 acidic residues[19]). The absence of this protein from the puffer fish genome is unlikely to be real. Column 8, Pix homologues were sought in genomes by BLAST with the Xenopus Pix2 sequence. The diagnostic feature of the Pix proteins was taken to be seven WD40 repeats plus homology in a coiled coil region in the C-terminus. The proteins were re-BLASTed again and their closest relatives were known Pix genes. The presence of the C-terminal coiled-coil region was confirmed using the program COILS (http://www.ch.embnet.org/software/COILS_form.html). The number of Pix homologues in genomes is shown in brackets. The similarity of Pix homologues is represented by a BLAST similarity score with the Xenopus Pix2 sequence.
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