Gene expression profile at the singe-cell level on human induced pluripotent stem cell (hiPSC) derived midbrain dopaminergic neurons

We optimised a new differentiation paradigm to produce midbrain dopaminergic neurons from hiPSCs. To characterise their identity, we performed scRNA-seq to investigate their gene expression and molecular identity. hiPSCs were differentiated using small molecule combination for 20 days to produce midbrain dopaminergic (mDA) precursor cells. These were then terminally differentiated into mDA neurons and grown for a further 28 days. The mDA monolayer was then dissociated into a single-cell suspension to perform scRNA-seq.