Example Files for pRESTO Jiang, He and Weinstein et al, 2013
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https://zenodo.org/record/14532471
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资源简介:
This example uses publicly available data from:
Lineage structure of the human antibody repertoire in response to influenza vaccination.Jiang N, He J, and Weinstein JA, et al.Sci Transl Med. 2013. 5(171):171ra19. doi:10.1126/scitranslmed.3004794.
Which may be downloaded from the NCBI Sequence Read Archive under accession ID: SRX190717. For this example, we will use the first 50,000 sequences of sample 43 (accession: SRR765688), which may downloaded downloaded using fastq-dump from the SRA Toolkit:
fastq-dump -X 50000 SRR765688
Primer and sample barcode (referred to as MID in Jiang, He and Weinstein et al, 2013) sequences are available in the published manuscript. This example assumes that the sample barcodes, forward primers (V-region), and reverse primers (C-region) have been extracted from the manuscript and placed into three corresponding FASTA files.
创建时间:
2024-12-19



