Comparative transcriptome and lipidome analyses reveal molecular chilling responses in chilling-tolerant sorghums

Chilling temperatures (0 to 15°C) are a major constraint for temperate cultivation of tropical-origin crops, including the cereal crop sorghum (Sorghum bicolor [L.] Moench). Northern Chinese sorghums have adapted to early-season chilling, but molecular mechanisms of chilling tolerance are unknown. We used RNA sequencing of seedlings to compare the chilling-responsive transcriptomes of a chilling-tolerant Chinese accession with a chilling-sensitive US reference line, and mass spectrometry to compare chilling-responsive lipidomes of four chilling-tolerant Chinese accessions with two US reference lines. Comparative transcriptomics revealed chilling-induced up-regulation of cold-response regulator C-repeat binding factor (CBF) transcription factor and genes involved in reactive oxygen detoxification, jasmonic acid (JA) biosynthesis, and lipid remodeling phospholipase Dα1 (PLDα1) gene in the chilling-tolerant Chinese line. Lipidomics revealed conserved chilling-induced increases in lipid uns...低温胁迫（0至15℃）是热带起源作物温带种植的主要限制性因素之一，其中包括谷类作物高粱（Sorghum bicolor [L.] Moench）。中国北方高粱已适应生长早期的低温环境，但目前其耐低温的分子机制仍不明晰。本研究通过对幼苗进行RNA测序（RNA sequencing），对比分析耐低温中国高粱种质与低温敏感型美国参考品系的低温响应转录组（transcriptome）；并借助质谱法（mass spectrometry），对比分析4份耐低温中国高粱种质与2份美国参考品系的低温响应脂质组（lipidome）。比较转录组学分析结果显示，在耐低温中国高粱品系中，低温诱导了冷响应调控因子C-重复结合因子（C-repeat binding factor, CBF）转录因子、活性氧解毒相关基因、茉莉酸（jasmonic acid, JA）生物合成基因以及脂质重塑相关磷脂酶Dα1（phospholipase Dα1, PLDα1）基因的上调表达。脂质组学分析则揭示了保守的低温诱导脂质不饱和（原文未完结）...