Genetic repertoire of Pseudomonas putida KT2440

Known for its versatile metabolism and capabilities in degrading organic pollutants, Pseudomonas putida KT2440 is emerging as a suitable microbial chassis for both metabolic engineering and environmental remediation applications. To understand the physiological and metabolic capabilities of a microorganism, the contemporary approach posits a look into its annotated genome. In particular, the types of genes present as well as their sequence constitute the basic information upon which metabolic pathways are discerned as well as genome scale metabolic network are constructed. To this end, the annotated genome sequence of P. putida KT2440 (Genbank accession number: NCBI Reference Sequence: NC_002947.4) was read by an in-house MATLAB software to enable the construction of a gene database comprising all annotated genes in the genome of the organism. Together with gene identifier, descriptor of the gene as well as its gene sequence were also curated in the gene database. The resource should be useful for researchers interested in the basic biology of P. putida KT2440 as well as metabolic engineers seeking to construct better biotechnology strains using the base strain as engineering chassis.