Alaska crustacean age determination study: calcein staining of calcified hard structures for three species, 2015-2017

These data are part of a crustacean age determination project. Specifically, these data describe fluorescence of gastric mill ossicles and eyestalks of red king crab, snow crab, and spot shrimp in relation to calcein exposure to evaluate chemical marking as a form of age and growth validation. The data set consists of one comma separated file exported from a Microsoft excel workbook. The data consists of 1. image green channel intensity data, 2. animal size, sex, molting data, shell condition, and 3. brachial chamber and calcein mark application data. Red king crab (Paralithodes camtschaticus) and Tanner crab (Chionoecetes bairdi) were collected near Juneau, Alaska between April 7, 2015 and April 14, 2015. Spot shrimp (Pandalus platyceros) were collected from Tenakee Inlet in Southeast Alaska during an Alaska Department of Fish and Game assessment survey in September 2015. Sex and shell condition were recorded at the time of collection. Carapace length/width and chela height were taken with digital calipers and recorded for each individual. All specimens were held at Lena Point Laboratory of the University of Alaska Fairbanks and held for the duration of the experiment.本数据集为甲壳类动物年龄鉴定项目的组成部分。具体而言，本数据集记录了红帝王蟹（red king crab）、雪蟹（snow crab）以及斑点虾（spot shrimp）的胃磨骨片与眼柄的荧光信号，相关实验通过钙黄绿素（calcein）暴露处理，旨在评估化学标记法在年龄与生长验证中的应用效果。本数据集包含1个从Microsoft Excel工作簿导出的逗号分隔值（CSV）文件，其数据分为三类：1. 图像绿色通道强度数据；2. 受试动物的体型、性别、蜕壳数据以及壳体状态信息；3. 鳃室与钙黄绿素标记施用相关数据。红帝王蟹（*Paralithodes camtschaticus*）与坦纳蟹（*Chionoecetes bairdi*）于2015年4月7日至4月14日期间，在阿拉斯加州朱诺附近海域采集获得；斑点虾（*Pandalus platyceros*）则于2015年9月，在阿拉斯加东南部特纳基湾开展的阿拉斯加渔猎部（Alaska Department of Fish and Game）评估调查中完成采集。样本采集时即记录了个体性别与壳体状态，使用数显游标卡尺测量每只个体的头胸甲长/宽以及螯高并予以记录。所有样本均饲养于阿拉斯加费尔班克斯大学莱纳角实验室，并在实验全程内完成饲养管理。