Regulatory elements coordinating initiation of chromosome replication to the Escherichia coli cell cycle

Bacteria replicate the genome on average once per generation, irrespective of growth condition-dependent changes in generation time and cell size. The strategy appears to be to initiate replication in a narrow chromosome-to-size ratio interval for each growth condition. To investigate how Escherichia coli accurately compares its size to the number of chromosomes, we perturbed some of the mechanisms proposed to achieve this precision and monitored how replication initiation was affected with time-lapse single-cell fluorescence microscopy. We found that the regulatory inactivation of DnaA-ATP (RIDA) mechanism, which converts the replication initiation molecule DnaA to its inactive form in proportion to the number of chromosomes, was the most important factor and that other previously described mechanisms have a less critical role. 

This dataset contains the raw data, analysis and code needed to generate the figures presented in the paper. The raw data consists of microscopy images, growth rate measurements, RT-qPCR, whole-genome sequencing and Rif-runout data. The analysis and code part consists of code and output from the analysis and post-processing.

细菌在平均每代中复制其基因组一次，无论代时和细胞大小因生长条件而发生的依赖性变化。该策略似乎是在每种生长条件下，在一个狭窄的染色体与大小比率的区间内启动复制。为了探究大肠杆菌如何精确地比较其大小与染色体数目，我们扰动了一些被提出以实现这种精度的机制，并利用时间序列单细胞荧光显微镜监测了复制启动随时间的变化。我们发现，将复制启动分子DnaA转化为非活性形式的DnaA-ATP（RIDA）调控失活机制，该机制按染色体数目成比例地转化DnaA，是至关重要的因素，而其他先前描述的机制则具有较次要的作用。本数据集包含了生成论文中展示的图表所需的原始数据、分析和代码。原始数据包括显微镜图像、生长速率测量、实时定量PCR、全基因组测序和Rif-runout数据。分析和代码部分包括分析代码和输出以及后续处理。