Myricetin on Triethylene Glycol Dimethacrylate Induced Matrix-Metalloproteinases Expression in Odontoblast cells Data

The following data presents the results obtained from each of the assays conducted in the study titled: Myricetin Modulate Matrix-Metalloproteinases Expression Induced by TEGDMA in human Odontoblast-like Cells. On the first sheet titled "Chromatography," the data corresponds to the evaluation of the presence of test substances in the culture medium, obtained through liquid cromatography coupled with mass spectrometry (LCMS). The second sheet titled "PCR" contains data related to the assessment of mRNA expression for MMP-2 and MMP-9, obtained through real-time polymerase chain reaction (RT-qPCR) from total RNA extracted from odontoblast-like cells exposed to 4-aminophenylmercuric acetate (APMA), hydrogen peroxide (H2O2), proanthocyanidin (PAC), myricetin (MYR), and triethylene glycol dimethacrylate (TEGDMA). On the third sheet titled "ELISA," you will find data regarding the assessment of MMP-2 and MMP-9 concentrations in supernatants from cultures of odontoblast-like cells exposed to Phorbol 12-myristate 13-acetate (PMA), H2O2, PAC, MYR, and TEGDMA. This information was obtained through an Enzyme-Linked Immunosorbent Assay (ELISA). The fourth sheet titled "MMP Activity Data" contains data pertaining to the assessment of MMP-2 and MMP-9 activity in supernatants from cultures of odontoblast-like cells exposed to ethylenediaminetetraacetic acid (EDTA), APMA, H2O2, PAC, MYR, and TEGDMA. This data was obtained through a Fluorescence Resonance Energy Transfer (FRET) assay. The data support the ability of myricetin to transcriptionally control the expression of MMP-2 and MMP-9 induced by contact with TEGDMA in odontoblast-like cells in a cellular model of dentin-odontoblastic cell coculture.本数据集收录了题为《杨梅素（Myricetin）调控三乙二醇二甲基丙烯酸酯（triethylene glycol dimethacrylate，TEGDMA）诱导的人成牙本质细胞样细胞（human Odontoblast-like Cells）的基质金属蛋白酶（Matrix-Metalloproteinases）表达》的研究中各项实验所得结果。首个命名为“色谱分析（Chromatography）”的工作表中，数据为通过液相色谱-质谱联用法（Liquid Chromatography-Mass Spectrometry，LC-MS）检测细胞培养介质中受试物质存在情况所得。第二个命名为“聚合酶链式反应（Polymerase Chain Reaction，PCR）”的工作表收录相关数据：采用实时荧光定量聚合酶链反应（real-time polymerase chain reaction，RT-qPCR），对经4-氨基苯汞乙酸盐（4-aminophenylmercuric acetate，APMA）、过氧化氢（hydrogen peroxide，H₂O₂）、原花青素（proanthocyanidin，PAC）、杨梅素（Myricetin，MYR）及三乙二醇二甲基丙烯酸酯（triethylene glycol dimethacrylate，TEGDMA）处理的人成牙本质细胞样细胞提取的总RNA，检测基质金属蛋白酶2（Matrix-Metalloproteinases 2，MMP-2）与基质金属蛋白酶9（Matrix-Metalloproteinases 9，MMP-9）的mRNA表达水平。第三个命名为“酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay，ELISA）”的工作表包含相关数据：通过酶联免疫吸附试验（ELISA），检测经佛波醇12-肉豆蔻酸酯13-乙酸酯（Phorbol 12-myristate 13-acetate，PMA）、H₂O₂、PAC、MYR及TEGDMA处理的人成牙本质细胞样细胞培养上清液中的MMP-2与MMP-9浓度。第四个命名为“基质金属蛋白酶活性数据（MMP Activity Data）”的工作表收录相关数据：通过荧光共振能量转移试验（Fluorescence Resonance Energy Transfer，FRET），检测经乙二胺四乙酸（ethylenediaminetetraacetic acid，EDTA）、APMA、H₂O₂、PAC、MYR及TEGDMA处理的人成牙本质细胞样细胞培养上清液中的MMP-2与MMP-9活性。本数据证实，在牙本质-成牙本质细胞共培养细胞模型中，杨梅素可对TEGDMA接触诱导的人成牙本质细胞样细胞内MMP-2与MMP-9的表达进行转录调控。