Summary of kinetic parameters.
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1Values shown are the average and standard deviation from triplicate experiments. Acetyl-CoA, acryloyl-CoA, butyryl-CoA, isobutyryl-CoA, malonyl-CoA and pentanoyl-CoA were also tried as substrates for CoA ester hydrolysis, but no activity was observed. In addition, acryloyl-CoA was not hydrated.2N.A. – No activity was detected. The limits for detection were 1.4×10−4 s−1 and 4.8×10−5 s−1 for CoA ester hydrolysis and MeSH release, respectively.
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2015-12-02



