Identification of CaMKIIβ binding lncRNAs in mouse hippocampus via RIP-seq

In order to screen for CaMKIIβ binding lncRNAs, we performed a RIP-seq analysis on mouse hippocampus. Potential CaMKIIβ binding RNAs are identified through differential expression analysis between CaMKIIβ RIP-seq results and the control groups, IgG and input. Differentially expressed genes are subsequently filtered by biotype and expression level. RIP assay is performed with CaMKIIβ (Cell Signaling Technology, 12716) and IgG (Cell Signaling Technology, I8140) antibodies. The resulting RNA is extracted and deep-sequenced. The data include three CaMKIIβ replicates, three IgG replicates and two replicates of input RNA expression levels.