Online resources: Portuguese Pinus nigra populations: genetic diversity, structure and relationships inferred by SSR markers

<b>Online resources 1</b>: Genetic diversity statistics assayed per Portuguese <i>P. nigra </i>population and SSR <i>locus</i>. Notes: na - observed number of alleles; ne - effective number of alleles (Kimura and Crow 1964); <i>I</i> - Shannon’s Information Index (Lewontin 1972); <i>h</i> – Nei’s gene diversity index (Nei 1973); Ho – observed heterozygosity; He – expected heterozygosity (Levene 1949); the F – fixation index; and s.d. – standard deviation. <b>Online resources 2</b>: Genetic differentiation coefficient (Gst) and differentiation index of allele frequencies Jost’s D estimated per SSR locus, and total mean value (± s.d.) determined in the 13 SSR markers. <b>Online resources 3</b>: PCoA based on the pooled SSR data using the Nei’s genetic distance coefficient (Nei 1978), showing the projection of the six Portuguese<i> P. nigra </i>populations Manteigas, Vale do Zêzere, Campeã, Vila Pouca de Aguiar, Caminha and Paredes de Coura and seven foreign samples representative of the subspecies <i>dalmatica</i>, <i>pallasiana</i>,<i> laricio</i>,<i> nigra</i>, <i>salzmannii.</i>The cumulative percentage of total variation explained by the first three coordinates was 74.50%. <b>Online resources 4</b>: SSR database obtained by thirteen SSR markers for six populations of Pinus nigra in Portugal. The populations are representative of the species distribution in Portugal: Manteigas and Vale do Zêzere (center), Campeã and Vila Pouca de Aguiar (north) and Paredes de Coura and Caminha (northwest).