SCD2-mediated monounsaturated fatty acid metabolism regulates cGAS-STING-dependent type I IFN in mouse CD4+ T cells

ACC1 is known to rate limitng enzyme of fatty acid biosynthesis. We report that modulation of fatty acid biosynthesis pathway increased tonic expression of anti-viral genes. RNA sequence was conducted to compare global gene expression between control, ACC1 inhibitor (TOFA)-treated, ACC1 KO and IFNb-treated Th1 cells. We found that inhibition or deletion of ACC1 resulted in the enhancement of anti-viral genes expression as comparable level to IFNb-treated Th1 cells. This study provides a framework for the relationship between fatty acid biosynthesis pathway and anti-viral responses in CD4 T cells. Overall design: RNA-seq was performed to investigate the role of fatty acid syntehsis pathway for gene expression In primay CD4+ T cells.