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Primer sets used for quantitative real-time PCR of differentially expressed genes (DEGs) identified from RNA-Seq between normal and stem browning samples of the mushroom Lentiula edodes

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jstagedata.jst.go.jp2024-10-04 更新2025-03-25 收录
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https://jstagedata.jst.go.jp/articles/dataset/Primer_sets_used_for_quantitative_real-time_PCR_of_differentially_expressed_genes_DEGs_identified_from_RNA-Seq_between_normal_and_stem_browning_samples_of_the_mushroom_i_Lentiula_edodes_i_/26829796/3
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Based on the results of the RNA-Seq analysis of black and normal stems in Lentinula edodes, quantitative reverse-transcription PCR (qRT-PCR) was performed to confirm the expression levels of 17 differentially expressed genes (DEGs) annotated as transcription factors and oxidoreductases. The primer sets used during this process are shown in this Table.

基于对香菇(Lentinula edodes)黑色与正常菌柄的RNA-Seq分析结果,执行了定量逆转录聚合酶链反应(qRT-PCR),以验证17个差异表达基因(DEGs)的表达水平,这些基因被注释为转录因子和氧化还原酶。此过程中使用的引物集详见下表。
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