Analysis of skin of keratinocyte-specific OTULIN-deficient mice by single-cell RNA-sequencing

In order to unravel the functional role of the linear deubiquitinase OTULIN, we performed single-cell RNA-sequencing on total skin of mice lacking OTULIN selectively in keratinocytes. Keratinocyte-specific OTULIN knock-out (KO) mice develop delineated inflammatory lesion. Through single-cell analysis on lesional and non-lesional skin of mice lacking OTULIN in keratinocytes, we could identify signalling pathways through which these inflammatory lesions appear, allowing us to get new insights on the molecular events that regulate skin homeostasis and mediate skin inflammation. The purpose of this study was to delineate the cellular and molecular changes that occur in the skin when linear deubiquitination is absent in keratinocytes. Total skin was harvested from wild-type (WT) skin and lesional and non-lesional keratinocyte-specific OTULIN knock-out (KO) mice. We FACS sorted live cells from single-cell suspensions of total skin.