Transcriptional profiling of CHB8 human embryonic stem cells with insulin receptor knockdown

While insulin receptor (IR) signaling is involved in the maintenance and proliferation of human embryonic stem cells (hESCs), its functional significance is poorly understood. To elucidate the role of IR in the pluripotency network, we knocked down INSR in CHB8 hESCs and performed RNA sequencing and differential expression analysis. We observe dysregulation of the AKT and ERK1/2 signalling pathways and downregulation of extracellular matrix genes. Overall design: RNA sequencing of CHB8 hESCs with shRNA knockdown of INSR.