Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells

We performed RNA-seq using pgEpiSCs at passage 30 (P30) and at P200 to further comprehensively assess the cellular properties. We subsequently employed the RNA-Seq dataset to focused on the expression level profiles of marker genes known to be involved in critical phases of myogenic lineage differentiation to determine if the differentiation scheme generates cells of the expected lineage at each stage. Overall design: Comparative gene expression profiling analysis of RNA-seq data for pgEpiSCs cells (pgEpiSCs-Low and pgEpiSCs-high), its myogenic lineage differentiation derivatives (pgEpiSCs-MPCs, pgEpiSCs-MCs) and myogenic differentiation of porcine muscle stem cells (MPC-Dif)