TrypOx, a novel eukaryotic homologue of the redox-regulated chaperone Hsp33 in Trypanosoma brucei

In this study, we describe a novel eukaryotic homologue of Hsp33 in Trypanosoma brucei.Bioinformatics analysis of the Hsp33 family suggested that eukaryotic pathogens belonging to the kinetoplastids, such as Trypanosoma cruzi,Trypanosoma brucei spp, and leishmaniasis had persevered Hsp33 homologues in their genomes. We showed that RNAi silencing of TrypOx led to a significant decrease in the survival of T.brucei under mild oxidative stress conditions,and then we adopted a proteomics-driven approach to investigatethe role of TrypOx in defining the oxidative stress response. Our results showed that depletion of TrypOx significantly altered the abundance of proteins mediating redox homeostasis,linking TrypOx with the antioxidant system and implying a protetcive role of Trypox against oxidative stress.