A novel kinase inhibitor for b-catenin mutant hepatocellular carcinoma [RNA-seq]

We screened HCC organoids to identify a CTNNB1-mutant HCC selective small molecule (WNTinib), with MOA depending on reactivation of cytoplasmic EZH2, leading to its nuclear translocation and selective transcriptional block of WNT targets We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 genetically different organoids at 3 time points. Comparative gene expression profiling analysis of RNA-seq data for MYC-CTNNB1 organoids +/- WNTinib vs MYC-Tp53 organoids +/- WNTinib (DMSO as controls)