A flexible and low-cost spatially resolved transcriptomics strategy making molecular tissue profiling a widely available solution

Spatially-resolved transcriptomics methodologies are revolutionizing our understanding of complex tissues, but their elevated costs represent still a bottle-neck for their democratization. In this work we present a low-cost strategy for manufacturing molecularly double-barcoded DNA arrays, enabling large-scale spatially-resolved transcriptomics studies. We applied this technique to spatially resolve gene expression in several human brain organoids, including the reconstruction of a 3-dimensional view from multiple consecutive sections, revealing gene expression divergencies throughout the tissue.

空间解析转录组学方法正在革新我们对复杂组织的理解，但其高昂的成本仍然是其普及化的瓶颈。在本研究中，我们提出了一种低成本策略，用于制造分子双重条形码DNA阵列，从而实现大规模空间解析转录组学研究。我们应用该技术对多个人类脑类器官中的基因表达进行了空间解析，包括从多个连续切片中重建三维视图，揭示了组织内基因表达的差异。