Transcription profiling of mouse placenta at different developmental time points

We used full genome microarrays to profile the full lifetime of the mouse placenta from embryonic day 8.5 (e8.5), at the time of chorioallantoic fusion, until postnatal day 0 (P0). For these samples, at each stage the fetal placenta and maternal decidual tissues were dissected and profiled separately (See series 1). For this experiment (Series 2), placental and decidual time course samples were normalized and modeled with two undissected (including placental and decidual tissue) e17 placentas to allow for scaling of values for comparison to the undissected placenta samples used in the publicly available mouse Gene Atlas dataset Experiment Overall Design: Mouse placentas were obtained from timed pregnant female mice at each time point, and fetal tissues were used to confirm embryo staging. For all dissected samples, fetal placenta and maternal decidual tissues were dissected and pooled separately for each litter prior to RNA extraction and hybridization on Affymetrix microarrays.本研究采用全基因组微阵列（full genome microarrays）分析小鼠胎盘从绒毛膜尿囊融合时期的胚胎第8.5天（e8.5）直至出生后第0天（P0）的整个生命周期的表达谱。针对该批次样本（系列1），每个发育阶段均将胎儿胎盘与母体蜕膜组织分离，并分别进行表达谱分析（详见系列1）。本实验（系列2）中，胎盘与蜕膜的时间序列样本经归一化处理后，以2份未解剖（包含胎盘及蜕膜组织）的e17胎盘作为参照进行数值标准化，以便与公开可用的小鼠基因图谱（Gene Atlas）数据集所使用的未解剖胎盘样本进行对比分析。实验整体设计：各时间点的小鼠胎盘均取自定时受孕的雌性小鼠，并通过胎儿组织确认胚胎发育分期。对于所有已解剖的样本，先将每窝小鼠的胎儿胎盘与母体蜕膜组织分别分离并混合，随后进行RNA提取，再在Affymetrix微阵列上完成杂交实验。