A self-propagating c-Met-SOX2 axis drives cancer-derived IgG signaling that promotes lung cancer cell stemness [RNA-seq]

Misplaced IgG expression in cancer cells has been implicated in exacerbated malignancy and poor clinical prognosis. Accumulating evidence indicate that a non-conventional sialylation modification is critical for the function of cancer-derived IgG, rendering the name sialylated cancer IgG (SIA-cIgG). However, our knowledge remains rudimentary about the functional role of SIA-cIgG in lung stemness maintenance. To investigate the mechanisms of SIA-cIgG in the regulation of lung stemness maintenance, we performed RNA-seq using established NCI-H520 cell lines sorted by FACS to have high or low expression of SIA-cIgG. This revealed that genes upregulated in SIA-cIgG high cells were associated with stem cell proliferation and maintenance. Overall design: Comparative gene expression profiling analysis of RNA-seq data for SIA-cIgG high/low NCI-H520 cells.