five

A collection of 16S reads for samples relating to pre-term infants

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https://www.ncbi.nlm.nih.gov/sra/ERP005870
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资源简介:
The NeoM (Neonatal Microbiota) Study is an ecological study investigating the microbiota of pre-term infants. Samples collected include faecal samples, skin swabs and swabs of infant incubators. Bacterial DNA was extracted from the samples using either MPBio FastDNA Spin kits or MPBio FastDNA Spin Kits for Soil and V3-V5 amplicons were generated using Bifidobacterium-optomised primers (Sim et al, 2012, PLOS ONE) and tagged with 12bp barcodes. These were then sequenced using a 454 Life Sciences GS FLX (Roche) following the Roche Amplicon Lib-L protocol. Both raw data (.sff files) and data denoised via ampliconnoise.py (as part of the Qiime pipeline) will be submitted here.
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2018-02-21
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