An insight into gill microbiome of Eastern Mediterranean wild fish by applying next generation sequencing

Fish gill tissue samples were processed and underwent PCR. Amplicons were sent to UIC Sequencing Core (Chicago, IL, USA) for library construction and sequencing. Sequence data was analyzed using the Dada2 pipeline using R package ‘dada2’. For each amplicon sequence variant (ASV), taxonomy (up to the species level) was inferred by alignment to the Silva non-redundant small subunit ribosomal RNA database. For data analysis and generation of figures, the online tool MicrobiomeAnalyst (https://www.microbiomeanalyst.ca/MicrobiomeAnalyst/home.xhtml) was used. Taxonomy labels were assigned using the SILVA taxonomic framework (https://www.arb-silva.de/documentation/silva-taxonomy/).