Zea mays Transcriptome or Gene expression

The experiment consisted of a field design containing two maize inbred lines Va35 and Mp313E. Va35 has yellow kernels and is susceptible to kernel infection by Aspergillus flavus (A. flavus). Mp313E is a white dent maize inbred line and was released primarily as a source of resistance to kernel infection by A. flavus. The test ears were inoculated with Aspergillus flavus and collected two days after inoculation. Non-inoculated ears collected 16 days after pollination were also used as a control. The microarray experimental design was a randomized complete block design with three replications. One microarray slide was used for each field plot. Each slide contained the inoculated and non-inoculated sub-treatments. A second slide for one replication of each genotype contained a dye swap for the inoculation treatment and three subsamples (dots) within the slide represented each contig. Keywords: Direct comparison Overall design: Two-condition experiment, inoculated vs. uninoculated maize ears for Va35 and Mp313E. Biological replicates: 3 control, 3 inoculated, 2 dye-swap, independently grown and harvested. One replicate per array.

本研究实验设计包含两个玉米自交系Va35和Mp313E。Va35品种籽粒呈黄色，易受黄曲霉（A. flavus）感染。Mp313E为白色硬粒玉米自交系，主要作为对黄曲霉（A. flavus）籽粒感染的抗性来源而发布。测试玉米穗在接种黄曲霉后两天进行采集。在授粉16天后采集的非接种玉米穗亦作为对照使用。微阵列实验设计为随机完全区组设计，包含三个重复。每个田间小区使用一个微阵列玻片。每个玻片包含接种和非接种亚处理。为每个基因型的每个重复制作第二个玻片，其中包含接种处理的染料交换，以及玻片内的三个子样本（点）代表每个连续序列。关键词：直接比较；整体设计：Va35和Mp313E玉米穗接种与未接种的双条件实验；生物重复：3个对照、3个接种、2个染料交换，独立种植和收获。每个阵列一个重复。